Protein Analysis Reagents
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Thermo Scientific™ Sulfo-EMCS (N-ε-maleimidocaproyl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of a water-soluble, 8-atom (9.4A) spacer arm.
Thermo Scientific™ Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) (sulfosuccinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)
Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based Renilla luciferase kit.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Green Renilla Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Green Renilla Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit
Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.
| Substrate | Coelenterazine, D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein G covalently bound to Dynabeads |
| Surface Functionality | Protein G |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein G |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Invitrogen™ DynaMag™-5 Magnet
The DynaMag-5 Magnet accommodates tubes from 11–16 mm in diameter (e.g., flow cytometry tubes and most blood collection tubes), with an optimal working volume of 1–5 mL.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Dynabeads™ M-270 Amine
These are hydrophilic Dynabeads™ (2.8 μm) with amine groups. The surface amino groups allow direct covalent binding of carbohydrates, glycoproteins and glycolipids through reductive amination of aldehydes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Type | Bead with Amine Group |
| Content And Storage | Store in refrigerator (2–8°C). |
| Purity or Quality Grade | Research Grade |
| Format | Beads in suspension |
| Surface Functionality | Amine |
| Sample Type | Any Sample Type |
| Diameter (Metric) | 2.8 μm |
| Shelf Life | 36 months from date of manufacture |
| High-throughput Compatibility | High-throughput Compatible |
| Elution Volume Min | 5 μL |
| Product Line | Dynabeads |
Thermo Scientific™ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ DTME (dithiobismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 436.37 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer Arm Length | 8.3 Å |
| Product Line | Pierce |
| PEGylated | No |
Invitrogen™ Dynabeads™ M-280 Sheep Anti-Mouse IgG
Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-μm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads M-280 Sheep Anti-Mouse IgG is supplied in PBS, pH 7.4 w/0.1% BSA, 0.02% NaN3 Store at 2°C to 8°C. |
| Description | Polyclonal sheep anti-mouse IgG covalently bound to Dynabeads |
| Color | Brown |
| Material | Polystyrene |
| Concentration | 10 mg/mL |
| For Use With (Application) | Protein Purification |
| High-throughput Compatibility | High-throughput Compatible |
| Regulatory Status | For Research Use Only |
| Ligand Type | Antibody |
| Type | Antibody Coated Bead |
| Target | Proteins |
| Format | Beads in suspension |
| Reactivity | Binds mouse IgG1, IgG2a and IgG2b. Low reactivity towards mouse IgG3 and IgM. |
| For Use With (Equipment) | KingFisher™ Sample Purification Systems, DynaMag™ Magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Shelf Life | 36 months from date of manufacture |
| Species | Sheep |
| Uniformity | Monosized 2.8 μm (CV <5%) |
Invitrogen™ Dynabeads™ MyOne™ Tosylactivated
When you wish to covalently couple antibodies, peptides, intact proteins, and functional enzymes to the surface of a magnetic bead choose Dynabeads Tosylactivated.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Type | Tosylactivated Bead |
| Content And Storage | Store at 2°C to 8°C. |
| Purity or Quality Grade | Research Grade |
| Format | Beads in solution |
| Surface Functionality | p-Toluene-Sulfonyl, Tosylactivated |
| Sample Type | Any Sample Type |
| High-throughput Compatibility | High-throughput Compatible |
| Elution Volume Min | 5 μL |
| Product Line | DYNAL, Dynabeads, MyOne |